Imperial College/31 August 2008
From 2008.igem.org
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====Wednesday==== | ====Wednesday==== | ||
*2nd trial of the ''B.subtilis'' growth curve and OD<sub>600</sub> vs c.f.u. experiment | *2nd trial of the ''B.subtilis'' growth curve and OD<sub>600</sub> vs c.f.u. experiment | ||
- | *Perform single colony PCR with the ''B.subtilis'' transformed yesterday | + | *Perform single colony PCR with the ''B.subtilis'' transformed yesterday using verifications primers. |
- | + | *Begin to try to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA. This includes the volume of transformed cells, amount of DNA, the density of competent cells and length of DNA incubation. | |
====Thursday==== | ====Thursday==== | ||
- | + | <br> | |
====Friday==== | ====Friday==== | ||
+ | *Carry on to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA |
Revision as of 00:25, 1 September 2008
31th August 2008Wet LabCloning
Monday
Tuesday
WednesdayTest orientation of inserts to find a correct Biobrick containing host to culture overnight for midiprepping Thursday - Friday
B.subtilisMonday
Tuesday
Wednesday
Thursday
Friday
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