Team:NTU-Singapore/Notebook/26 August 2008
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(New page: =Tuesday 26 August= *Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check. *Hung: digestional check:<br/>Results: **Among 4 samples of Lsr...)
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(New page: =Tuesday 26 August= *Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check. *Hung: digestional check:<br/>Results: **Among 4 samples of Lsr...)
Newer edit →
Revision as of 11:47, 2 September 2008
Tuesday 26 August
- Morning: Lu Chao: minipreps of 6 colonies inoculated on Monday and prepared 2% gel for later gel check.
- Hung: digestional check:
Results:- Among 4 samples of LsrA-RBS-Lysis-Term, 1 sample showed the correct bands: 2kb vector + 450bp insert (total length of LsrA+RBS+Lysis+Term). Our detection+lysis system is done!!
- 2 samples of LacI-RBS-E7-Term showed wrong bands. However, ligation mixture for this was still not used up. We would transform the remaining mixture into top10 cells on Friday.