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EPF-Lausanne/2 September 2008
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==Ligations== | ==Ligations== | ||
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| + | F2 had a lot of cells growing, but also on the control. We pick a couple of colonies and make liquid cell cultures anyway, we will see on the gel later if we got lucky and picked one with the insert. | ||
| + | But we restart also a new digestion/ligation/transformation of F2. | ||
Latest revision as of 13:28, 3 September 2008
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We made new chemically competent cells
Ligations
F2 had a lot of cells growing, but also on the control. We pick a couple of colonies and make liquid cell cultures anyway, we will see on the gel later if we got lucky and picked one with the insert. But we restart also a new digestion/ligation/transformation of F2.
