Team:Paris/September 2

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(Difference between revisions)
(→Ligation L172 (rbs-FlhDC in mRFP-LVA-tripart-pSB1A3))
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
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{{Paris/Calendar_Links|September 1|September 3}}
{{Paris/Calendar_Links|September 1|September 3}}
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=Ligation L173 ('''rbs-TetR''' in '''mRFP-LVA-tripart'''-pSB1A3)=
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=Construction of rbs-TetR-mRFP-LVA-tripart=
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'''Ligation L173'''  
'''Samples used'''
'''Samples used'''
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*Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
*Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
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=Construction of rbs-FlhDC-mRFP-LVA-tripart=
=Construction of rbs-FlhDC-mRFP-LVA-tripart=

Revision as of 20:18, 3 September 2008

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Construction of rbs-TetR-mRFP-LVA-tripart

Ligation L173

Samples used

Name Digestion number Digested by DNA concentration
rbs-TetR D112 EcoRI & SpeI (insert) ~20 µg/mL
mRFP-LVA-tripart D187 EcoRI & XbaI (vector) 16 µg/mL


Reaction mixtures

Control Sample
3:1 (insert:vector) ratio 4:1 (insert:vector) ratio
D112 (insert) 0 µL 1,4 µL 1,9 µL
D187 (vector) 2,5 µL 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
water 14,5 µL 13,1 µL 12,6 µL
  • Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).


Construction of rbs-FlhDC-mRFP-LVA-tripart

Samples used

Name Digestion number Digested by DNA concentration
rbs-FlhDC D186 EcoRI & SpeI (insert) ~24 µg/mL
mRFP-LVA-tripart D187 EcoRI & XbaI (vector) 16 µg/mL


Reaction mixtures

Control Sample
D186 (insert) 0 µL 1 µL
D187 (vector) 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL
water 14,5 µL 13,5 µL
  • Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction).
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