Team:University of Lethbridge/Notebook/Project2August

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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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===August 1, 2008===
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====Nathan Puhl, Roxanne====
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Riboswitch 20 uL PCR. Set up 4 reactions (25 uL for each total volume). 1 uL or 1/100 pTopp and 1 uL or H1/100 PCR from July 28, 2008.
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PCR conditions:
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A. Initial denaturation: 98 C (3 min)
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B. -Denaturation: 98 C (10 sec)
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    - Annealing: 55 C (30 sec)
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    -Extension: 72 C (15 sec)
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    -30 cycles
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C. Final extension: 72 C (7 min)
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===August 2, 2008===
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====Nathan Puhl, Roxanne====
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Ran riboswitch (Aug. 1, 2008) on 3% agarose gel. Results are in the hard copy lab notebook. Looks like 76 bp band will extract.

Revision as of 23:12, 3 September 2008

Back to The University of Lethbridge Main Notebook


Contents

August 1, 2008

Nathan Puhl, Roxanne

Riboswitch 20 uL PCR. Set up 4 reactions (25 uL for each total volume). 1 uL or 1/100 pTopp and 1 uL or H1/100 PCR from July 28, 2008.

PCR conditions: 

A. Initial denaturation: 98 C (3 min)
B. -Denaturation: 98 C (10 sec)
    - Annealing: 55 C (30 sec)
    -Extension: 72 C (15 sec)
    -30 cycles
C. Final extension: 72 C (7 min)

August 2, 2008

Nathan Puhl, Roxanne

Ran riboswitch (Aug. 1, 2008) on 3% agarose gel. Results are in the hard copy lab notebook. Looks like 76 bp band will extract.

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