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Alberta NINT/21 August 2008
From 2008.igem.org
(Difference between revisions)
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-to run on gel and check if in fact both plasmids inside the cell are low copy plasmids, bands should be about the same brightness | -to run on gel and check if in fact both plasmids inside the cell are low copy plasmids, bands should be about the same brightness | ||
Ran on gel and found that the band intensities were not the same. | Ran on gel and found that the band intensities were not the same. | ||
| - | [[Image: | + | [[Image:UVP0343.jpg]] |
Analyzed LacZ Assay Data | Analyzed LacZ Assay Data | ||
[[Media:Alberta_NINT_LacZ_assay_August_22_08.xls]] | [[Media:Alberta_NINT_LacZ_assay_August_22_08.xls]] | ||
Revision as of 03:15, 4 September 2008
lab work (SD):
Ligated TA2+(PCR)/B+N (NR) into K102031/B+N (GP).
Transformed into XL1-B cells and incubated overnight at 37 C.
lab work (JD):
Digested mini-prep of cotransformed cells with EcoR1
-to run on gel and check if in fact both plasmids inside the cell are low copy plasmids, bands should be about the same brightness
Ran on gel and found that the band intensities were not the same.
Analyzed LacZ Assay Data
Media:Alberta_NINT_LacZ_assay_August_22_08.xls
