User:University of Washington/5 September 2008

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==LuxR from AraC and TetR(Faifan)==
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- measured Absorbance of the overnight culture at 660 nm = 1.5
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- diluted 60 ul culture into 5.40 ml Tsy+KAN. Absorbance at 660 nm = 0.035
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- incubated 37 degree Celsius for 2.5 hrs, Absorbance at 600 nm = 0.7
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- diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165
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- set up 96 well plate reader experiment. The total volume in each well was 200 ul.
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{|
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|-
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!!!rowspan=5|Arabinose(%), 10%Arabinose added (ul)
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|-
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!!!0, 0!!0.05, 1!!0.1, 2!!0.50, 10!!1.00, 20
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|}
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Back to [[Team:University_of_Washington/Notebook#Notebook]]
Back to [[Team:University_of_Washington/Notebook#Notebook]]

Revision as of 23:45, 5 September 2008

LuxR from AraC and TetR(Faifan)

- measured Absorbance of the overnight culture at 660 nm = 1.5

- diluted 60 ul culture into 5.40 ml Tsy+KAN. Absorbance at 660 nm = 0.035

- incubated 37 degree Celsius for 2.5 hrs, Absorbance at 600 nm = 0.7

- diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165

- set up 96 well plate reader experiment. The total volume in each well was 200 ul.

Arabinose(%), 10%Arabinose added (ul)
0, 00.05, 10.1, 20.50, 101.00, 20


Back to Team:University_of_Washington/Notebook#Notebook

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