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User:University of Washington/5 September 2008
From 2008.igem.org
(Difference between revisions)
(→LuxR from AraC and TetR(Faifan)) |
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- diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165 | - diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165 | ||
| - | - set up 96 well plate reader experiment | + | - set up 96 well plate reader experiment: |
| + | *5x5, 160 ul culture | ||
| - | {| | + | {| style="text-align:center" border=1 |
| + | |||
| + | !colspan=2 rowspan=2| !!colspan=5|Arabinose(%), 10%Arabinose added (ul) | ||
|- | |- | ||
| - | !!! | + | !0%, 0 ul!!0.05%, 1ul!!0.1%, 2ul!!0.50%, 10ul!!1.00%, 20ul |
|- | |- | ||
| - | !!!0, | + | !rowspan=5 valign=center|aTc(ng/ml), 10ug/ml-aTc added(ul)!!0, 0ul|||||||||| |
| - | + | |- | |
| + | !50, 1ul|||||||||| | ||
| + | |- | ||
| + | !100, 2ul|||||||||| | ||
| + | |- | ||
| + | !500, 10ul|||||||||| | ||
| + | |- | ||
| + | !1000, 20 ul|||||||||| | ||
|} | |} | ||
| + | *added dH2O to final the volume to 200 ul | ||
| + | *added 40 ul of mineral oil to each well to protect evaporation | ||
| + | *set the machine to run 24 hrs, read every 10 mins | ||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 00:01, 6 September 2008
LuxR from AraC and TetR(Faifan)
- measured Absorbance of the overnight culture at 660 nm = 1.5
- diluted 60 ul culture into 5.40 ml Tsy+KAN. Absorbance at 660 nm = 0.035
- incubated 37 degree Celsius for 2.5 hrs, Absorbance at 600 nm = 0.7
- diluted 1 ml culture into 5 ml Tsy+Kan, OD@600nm = 0.165
- set up 96 well plate reader experiment:
- 5x5, 160 ul culture
| Arabinose(%), 10%Arabinose added (ul) | ||||||
|---|---|---|---|---|---|---|
| 0%, 0 ul | 0.05%, 1ul | 0.1%, 2ul | 0.50%, 10ul | 1.00%, 20ul | ||
| aTc(ng/ml), 10ug/ml-aTc added(ul) | 0, 0ul | |||||
| 50, 1ul | ||||||
| 100, 2ul | ||||||
| 500, 10ul | ||||||
| 1000, 20 ul | ||||||
- added dH2O to final the volume to 200 ul
- added 40 ul of mineral oil to each well to protect evaporation
- set the machine to run 24 hrs, read every 10 mins
Back to Team:University_of_Washington/Notebook#Notebook
