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Team:Paris/Modeling/f7
From 2008.igem.org
(Difference between revisions)
| Line 31: | Line 31: | ||
|[expr(pFlgB)] | |[expr(pFlgB)] | ||
|expression rate of <br> pFlgB '''with RBS E0032''' | |expression rate of <br> pFlgB '''with RBS E0032''' | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''? | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''? | ||
|- | |- | ||
| Line 37: | Line 37: | ||
|dilution-degradation rate <br> of GFP(mut3b) | |dilution-degradation rate <br> of GFP(mut3b) | ||
|s<sup>-1</sup> | |s<sup>-1</sup> | ||
| - | | | + | |0.0198 |
|- | |- | ||
|[GFP] | |[GFP] | ||
| Line 65: | Line 65: | ||
|β<sub>57</sub> | |β<sub>57</sub> | ||
|production rate of FlhDC-pFlgB '''with RBS E0032''' <br> β<sub>57</sub> | |production rate of FlhDC-pFlgB '''with RBS E0032''' <br> β<sub>57</sub> | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
| | | | ||
|- | |- | ||
| - | |(K<sub>51</sub>/{coef<sub>flhDC</sub>} | + | |(K<sub>51</sub>/{coef<sub>flhDC</sub>}) |
|activation constant of FlhDC-pFlgB <br> K<sub>51</sub> | |activation constant of FlhDC-pFlgB <br> K<sub>51</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
| Line 84: | Line 84: | ||
| | | | ||
|- | |- | ||
| - | |(K<sub>52</sub>/{coef<sub>fliA</sub>} | + | |(K<sub>52</sub>/{coef<sub>fliA</sub>}) |
|activation constant of FliA-pFlgB <br> K<sub>52</sub> | |activation constant of FliA-pFlgB <br> K<sub>52</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
Revision as of 13:32, 6 September 2008
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFlgB)] | expression rate of pFlgB with RBS E0032 | nM.min-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | 0.0198 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β57 | production rate of FlhDC-pFlgB with RBS E0032 β57 | nM.min-1 | |
| (K51/{coefflhDC}) | activation constant of FlhDC-pFlgB K51 | nM | |
| n51 | complexation order of FlhDC-pFlgB n51 | no dimension | |
| β58 | production rate of FliA-pFlgB with RBS E0032 β52 | nM.s-1 | |
| (K52/{coeffliA}) | activation constant of FliA-pFlgB K52 | nM | |
| n52 | complexation order of FliA-pFlgB n52 | no dimension |
Then, if we have time, we want to verify the expected relation
