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Team:Paris/Modeling/f8
From 2008.igem.org
(Difference between revisions)
| Line 31: | Line 31: | ||
|[expr(pFlhB)] | |[expr(pFlhB)] | ||
|expression rate of <br> pFlhB '''with RBS E0032''' | |expression rate of <br> pFlhB '''with RBS E0032''' | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''? | |see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''? | ||
|- | |- | ||
|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
|dilution-degradation rate <br> of GFP(mut3b) | |dilution-degradation rate <br> of GFP(mut3b) | ||
| - | | | + | |min<sup>-1</sup> |
| - | | | + | |0.0198 |
|- | |- | ||
|[GFP] | |[GFP] | ||
| Line 65: | Line 65: | ||
|β<sub>6</sub> | |β<sub>6</sub> | ||
|production rate of FlhDC-pFlhB '''with RBS E0032''' <br> β<sub>6</sub> | |production rate of FlhDC-pFlhB '''with RBS E0032''' <br> β<sub>6</sub> | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
| | | | ||
|- | |- | ||
| - | |(K<sub>4</sub>/{coef<sub>flhDC</sub>} | + | |(K<sub>4</sub>/{coef<sub>flhDC</sub>}) |
|activation constant of FlhDC-pFlhB <br> K<sub>4</sub> | |activation constant of FlhDC-pFlhB <br> K<sub>4</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
| Line 81: | Line 81: | ||
|β<sub>14</sub> | |β<sub>14</sub> | ||
|production rate of FliA-pFlhB '''with RBS E0032''' <br> β<sub>14</sub> | |production rate of FliA-pFlhB '''with RBS E0032''' <br> β<sub>14</sub> | ||
| - | |nM. | + | |nM.min<sup>-1</sup> |
| | | | ||
|- | |- | ||
| - | |(K<sub>10</sub>/{coef<sub>fliA</sub>} | + | |(K<sub>10</sub>/{coef<sub>fliA</sub>}) |
|activation constant of FliA-pFlhB <br> K<sub>10</sub> | |activation constant of FliA-pFlhB <br> K<sub>10</sub> | ||
| - | |nM | + | |nM |
| | | | ||
|- | |- | ||
Revision as of 13:37, 6 September 2008
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFlhB)] | expression rate of pFlhB with RBS E0032 | nM.min-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β6 | production rate of FlhDC-pFlhB with RBS E0032 β6 | nM.min-1 | |
| (K4/{coefflhDC}) | activation constant of FlhDC-pFlhB K4 | nM | |
| n4 | complexation order of FlhDC-pFlhB n4 | no dimension | |
| β14 | production rate of FliA-pFlhB with RBS E0032 β14 | nM.min-1 | |
| (K10/{coeffliA}) | activation constant of FliA-pFlhB K10 | nM | |
| n10 | complexation order of FliA-pFlhB n10 | no dimension |
Then, if we have time, we want to verify the expected relation
