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Template:Team:UC Berkeley/Notebook/Molly construction
From 2008.igem.org
(Difference between revisions)
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| + | '''== Molly's Construction Files ==''' | ||
| + | |||
<pre> | <pre> | ||
Construction of {xis!} Biobrick Part K112200 | Construction of {xis!} Biobrick Part K112200 | ||
| Line 6: | Line 8: | ||
---------------------------------------- | ---------------------------------------- | ||
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag | mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag | ||
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc |
</pre> | </pre> | ||
| Line 16: | Line 18: | ||
---------------------------------------- | ---------------------------------------- | ||
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg | mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg | ||
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc |
</pre> | </pre> | ||
| Line 46: | Line 48: | ||
---------------------------------------- | ---------------------------------------- | ||
mea017 Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg | mea017 Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg | ||
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc |
</pre> | </pre> | ||
| Line 56: | Line 58: | ||
---------------------------------------- | ---------------------------------------- | ||
mea018 Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg | mea018 Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg | ||
| - | mea002 Reverse Biobricking of {xis!} | + | mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc |
</pre> | </pre> | ||
| Line 185: | Line 187: | ||
Product is pBca1256-K112219 | Product is pBca1256-K112219 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea013 Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc | + | mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc |
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | ||
</pre> | </pre> | ||
| Line 195: | Line 197: | ||
Product is pBca1256-K112220 | Product is pBca1256-K112220 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea015 Forward Biobricking of {<ihfB!}ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc | + | mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc |
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg | ||
</pre> | </pre> | ||
| Line 205: | Line 207: | ||
Product is pBca1256-K112221 | Product is pBca1256-K112221 | ||
---------------------------------------- | ---------------------------------------- | ||
| - | mea013 Forward Biobricking of {ihfB!}ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc | + | mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc |
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc | mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc | ||
</pre> | </pre> | ||
Revision as of 17:17, 6 June 2008
== Molly's Construction Files ==
Construction of {xis!} Biobrick Part K112200
PCR mea001/mea002 on pAH57 (230 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112200
----------------------------------------
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc
Construction of {<xis!} Biobrick Part K112201
PCR mea003/mea002 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112201
----------------------------------------
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc
Construction of {xis>} Biobrick Part K112202
PCR mea001/mea004 on pAH57 (227 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112202
----------------------------------------
mea001 Forward Biobricking of {xis!} ccataGAATTCatgAGATCTatgtacttgacacttcaggag
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {<xis>} Biobrick Part K112203
PCR mea003/mea004 on pAH57 (224 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112203
----------------------------------------
mea003 Forward Biobricking of {<xis!} ccataGAATTCatgAGATCTtacttgacacttcaggagtg
mea004 Reverse Biobricking of {xis>} cgttaGGATCCtgacttcgccttcttcccatttc
Construction of {a~xis!} Biobrick Part K112204
PCR mea017/mea002 on pAH57 (234 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112204
----------------------------------------
mea017 Forward Biobricking of {a~xis!}ccataGAATTCatgAGATCTtgcgatgtacttgacacttcagg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc
Construction of {rbs.xis!} Biobrick Part K112205
PCR mea018/mea002 on pAH57 (249 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112205
----------------------------------------
mea018 Forward Biobricking of {rbs.xis!}ccataGAATTCatgAGATCTtaattgcggagactttgcgatg
mea002 Reverse Biobricking of {xis!} cgttaGGATCCtcatgacttcgccttcttcccc
Construction of {int!} Biobrick Part K112207
PCR mea005/mea006 on pAH57 (1102 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112207
----------------------------------------
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {<int!} Biobrick Part K112208
PCR mea007/mea006 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112208
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {int>} Biobrick Part K112209
PCR mea005/mea008 on pAH57 (1099 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112209
----------------------------------------
mea005 Forward Biobricking of {int!} ccataGAATTCatgAGATCTatgggaagaaggcgaagtcatg
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {<int>} Biobrick Part K112210
PCR mea007/mea008 on pAH57 (1096 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112210
----------------------------------------
mea007 Forward Biobricking of {<int!} ccataGAATTCatgAGATCTggaagaaggcgaagtcatgagc
mea008 Reverse Biobricking of {int>} cgttaGGATCCtttgatttcaattttgtcccactccc
Construction of {a~int!} Biobrick Part K112211
PCR mea019/mea006 on pAH57 (1106 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112211
----------------------------------------
mea019 Forward Biobricking of {a~int!}ccataGAATTCatgAGATCTagaaatgggaagaaggcgaag
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {rbs.int!} Biobrick Part K112212
PCR mea020/mea006 on pAH57 (1120 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112212
----------------------------------------
mea020 Forward Biobricking of {rbs.int!}ccataGAATTCatgAGATCTttttgaagaggatcagaaatggg
mea006 Reverse Biobricking of {int!} cgttaGGATCCttatttgatttcaattttgtcccactccc
Construction of {ihfA!} Biobrick Part K112213
PCR mea009/mea010 on MG1655 (331 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112213
----------------------------------------
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {<ihfA!} Biobrick Part K112214
PCR mea011/mea010 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112214
----------------------------------------
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfA>} Biobrick Part K112215
PCR mea009/mea012 on MG1655 (328 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112215
----------------------------------------
mea009 Forward Biobricking of {ihfA!}ccataGAATTCatgAGATCTatggcgcttacaaaagctgaaatg
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
Construction of {<ihfA>} Biobrick Part K112216
PCR mea011/mea012 on MG1655 (325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112216
----------------------------------------
mea011 Forward Biobricking of {<ihfA!}ccataGAATTCatgAGATCTgcgcttacaaaagctgaaatgtc
mea012 Reverse Biobricking of {ihfA>} cgttaGGATCCctcgtctttgggcgaagcg
Construction of {a~ihfA!} Biobrick Part K112217
PCR mea021/mea010 on MG1655 (335 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112217
----------------------------------------
mea021 Forward Biobricking of {a~ihfA!}ccataGAATTCatgAGATCTacctatggcgcttacaaaagc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {rbs.ihfA!} Biobrick Part K112218
PCR mea022/mea010 on MG1655 (350 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112218
----------------------------------------
mea022 Forward Biobricking of {rbs.ihfA!}ccataGAATTCatgAGATCTtcattgagggattgaacctatggcgc
mea010 Reverse Biobricking of {ihfA!} cgttaGGATCCttactcgtctttgggcgaagcg
Construction of {ihfB!} Biobrick Part K112219
PCR mea013/mea014 on MG1655 (316 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112219
----------------------------------------
mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {<ihfB!} Biobrick Part K112220
PCR mea015/mea014 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112220
----------------------------------------
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {ihfB>} Biobrick Part K112221
PCR mea013/mea016 on MG1655 (313 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112221
----------------------------------------
mea013 Forward Biobricking of {ihfB!} ccataGAATTCatgAGATCTatgaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {<ihfB>} Biobrick Part K112222
PCR mea015/mea016 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112222
----------------------------------------
mea015 Forward Biobricking of {<ihfB!} ccataGAATTCatgAGATCTaccaagtcagaattgatagaaagacttgccaccc
mea016 Reverse Biobricking of {ihfB>} cgttaGGATCCaccgtaaatattggcgcgatc
Construction of {a~ihfB!} Biobrick Part K112223
PCR mea023/mea014 on MG1655 (310 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112223
----------------------------------------
mea023 Forward Biobricking of {a~ihfB!}ccataGAATTCatgAGATCTaatcatgaccaagtcagaattgatag
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
Construction of {rbs.ihfB!} Biobrick Part K112224
PCR mea024/mea014 on MG1655 (337 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112224
----------------------------------------
mea024 Forward Biobricking of {rbs.ihfB!}ccataGAATTCatgAGATCTctttaaggaaccggaggaatcatg
mea014 Reverse Biobricking of {ihfB!} cgttaGGATCCttaaccgtaaatattggcgcgatcg
