Team:University of Ottawa/5 June 2008

From 2008.igem.org

(Difference between revisions)
(Today in the Lab)
(Today in the Lab)
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==Today in the Lab==
==Today in the Lab==
'''Dan, Matt'''
'''Dan, Matt'''
 +
:'''igestion'''
::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
::<li>We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
 +
:'''Gel Results'''
::<li>Gel was unsuccessful for 1A but results for other plasmids worked well.
::<li>Gel was unsuccessful for 1A but results for other plasmids worked well.
::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
::<li>We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
-
::<li>Colonies were then inoculated using LB + solution and left overnight.
+
::<li>Colonies were then inoculated using LB + Amp solution and left overnight.
 +
:'''Glycerol Stock'''
 +
::<li>Glycerol Stock was performed for plasmids 7/8 and 0.

Revision as of 18:25, 6 June 2008

Today in the Lab

Dan, Matt

igestion
  • We decided to use different enzymes - Bam HI + EcoRI for 8/7 and xhoI for 0/1.
  • Gel Results
  • Gel was unsuccessful for 1A but results for other plasmids worked well.
  • We decided to choose 6 new colonies for the 1A plasmid and perform a miniprep on them.
  • Colonies were then inoculated using LB + Amp solution and left overnight.
  • Glycerol Stock
  • Glycerol Stock was performed for plasmids 7/8 and 0.