Alberta NINT/30 May 2008
From 2008.igem.org
(Difference between revisions)
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- | lab work (SD): DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol. This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004. | + | lab work (SD): |
- | + | ||
- | lab work (JD): Counted colonies on ligation plates: negative plate:0, Positive plate:2 | + | DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol. |
+ | This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004. | ||
+ | |||
+ | |||
+ | lab work (JD): | ||
+ | |||
+ | Counted colonies on ligation plates: negative plate:0, Positive plate:2 | ||
+ | because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow |
Revision as of 21:46, 6 June 2008
lab work (SD):
DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol. This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
lab work (JD):
Counted colonies on ligation plates: negative plate:0, Positive plate:2 because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow