Imperial College/11 September 2008

(Difference between revisions)

Revision as of 19:03, 11 September 2008

Tracked 23 cells (most were just running)
The figure below shows the velocity (per frame) distribution of cells.
We found that there were very few cells which were actually tumbling. Since we are going to use EpsE to control swimming motility of B. subtilis by stopping it, we have considered modelling it using 2 methods:
- Velocity distribution: In this method, we track cells, and obtain a plot of run velocity of the wild type cells. Upon known EpsE expression, we obtain another plot and compare the velocity distributions. This method focuses on EpsE acting to slow down the motility of B. subtilis.
- % of cells with swimming motility: In this method, we do not track cells, but count them over several frames to obtain the fraction of cells which are motile within a population. In this case we assume that when we combine the number of videos captured, it is representative of the entire population. If we see a significant drop in % of motile cells, we thus confirm EpsE's capability of disengaging the bacteria flagellar from its motor.

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 ==Dry Lab==
 *Tracked 23 cells (most were just running)
+*The figure below shows the velocity (per frame) distribution of cells.
+*We found that there were very few cells which were actually tumbling. Since we are going to use EpsE to control swimming motility of ''B. subtilis'' by stopping it, we have considered modelling it using 2 methods:
+**Velocity distribution: In this method, we track cells, and obtain a plot of run velocity of the wild type cells. Upon known EpsE expression, we obtain another plot and compare the velocity distributions. This method focuses on EpsE acting to slow down the motility of ''B. subtilis''.
+**% of cells with swimming motility: In this method, we do not track cells, but count them over several frames to obtain the fraction of cells which are motile within a population. In this case we assume that when we combine the number of videos captured, it is representative of the entire population. If we see a significant drop in % of motile cells, we thus confirm EpsE's capability of disengaging the bacteria flagellar from its motor.
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