1. Gel electrophoresis (PCR products) and isolation from proper bands
2. Digest with HindIII and SalI
3. Digest of pMPM-T5 + AID with HindIII and SalI
4. Clean-up products of p.2 and p.3 reactions
5. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion)
6. Transformation of E.coli TOP10 with ligation product
7. Transformants onto LB+tetracycline
8. Digest of pMPM-T5 + transcription fusion with EcoRHI and HindIII
9. Use of T4 polymerase for blunting
10. Gel electrophoresis
11. Isolation from proper band
12. Ligation of product p.11 (pMPM-T5 + T7 RNA-polymerase)
13. Transformation of E.coli TOP10 with ligation product
14. Transformants onto LB + tetracycline