Team:Warsaw/Calendar-Main/10 June 2008

From 2008.igem.org

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<p>1. Plasmid isolation from previous day's colonies.<br>
<p>1. Plasmid isolation from previous day's colonies.<br>
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2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and SalI<br>
+
2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI<br>
-
3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion with HindIII and EcoRI<br>
+
3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI<br>
-
4. Control digest of pMPM-T5 + T7 RNA-polimerase with EcoRI<br>
+
4. Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI<br>
-
5. Control digest of pMPM-T5 + T7 RNA-polimerase with BamHI and XhoI<br>
+
5. Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI<br>
6. DNA electrophoresis of digestion products<br>
6. DNA electrophoresis of digestion products<br>

Revision as of 21:15, 14 September 2008

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1. Plasmid isolation from previous day's colonies.
2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI
3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI
4. Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI
5. Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI
6. DNA electrophoresis of digestion products
7. Choice of proper colonies of transformants

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