Team:Hawaii/PCC6803 Cryostock Thaw Test

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Revision as of 11:34, 7 June 2008

Contents

Experiment Title

  • Thaw PCC6803 -80C deep frozen stock to ensure that our cryogenic protocol works. This will ensure that we will have re animatable Synechocystis cells from deep freeze in-case everything dies.

Methods

  • 2008.05.09 Cryogenically frozen PCC6803 cells using the methods prescribed here.
  • 2008.06.04 Thawed frozen stock from 5/9, spun down, added fresh BG-11 media (+thiosulfate, +TES pH 8.0 adjusted). Incubate in cryotube in complete darkness (aluminum foil wrapped) @ 26C for two days.
  • 2008.06.06
    • Method #1: Plated cryo-tube content using streak and hockey puck methods
    • Method #2: Liquid culture by adding 500 uL cryo-tube content to 10 mL BG-11 in T75 flat rectangular tissue culture flask. Shaken at 100rpm using Innova 2050.

Results

  • (check back after a week from 6/6/08)

Discussion

  • Insanity is doing the same thing over and over again and expecting different results. - Albert Einstein


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