Frozen glycerol stock
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(New page: * Take 2mls of ON culture and pipette into a sterilised cryo tube. * Add 0.5ml of autoclaved 50% glycerol solution * Vortex briefly to mix * Store at -80˚C)
Newer edit →
(New page: * Take 2mls of ON culture and pipette into a sterilised cryo tube. * Add 0.5ml of autoclaved 50% glycerol solution * Vortex briefly to mix * Store at -80˚C)
Newer edit →
Revision as of 15:42, 16 September 2008
- Take 2mls of ON culture and pipette into a sterilised cryo tube.
- Add 0.5ml of autoclaved 50% glycerol solution
- Vortex briefly to mix
- Store at -80˚C