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Colony stab
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| - | ==Making Overnight Cultures from Agar | + | ==Making Overnight Cultures from Agar Colonies== |
* Pipette 10mL LB into red-capped culture tubes or conical shake flasks. | * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. | ||
Latest revision as of 11:43, 18 September 2008
Making Overnight Cultures from Agar Colonies
- Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
- Using an autoclaved toothpick, gently touch the point to a single colony.
- Drop the toothpick point down into the LB.
- Incubate overnight at 37°C whilst shaking.
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