Team:Warsaw/Calendar-Main/24 June 2008

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<p>
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'''Preparation of A+AT construct'''<br>
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'''Preparation of pMPMT5-AID+AIDT7 construct'''<br>
1. PCR - translation fusion: AID + T7 RNA-polimerase <br>
1. PCR - translation fusion: AID + T7 RNA-polimerase <br>
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2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br
2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br
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<h3>PCR results of translational fusion for the A+AT construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>
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<h3>PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>
https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg
https://static.igem.org/mediawiki/2008/0/08/Tranlacyjna_pcr_29_05_2008.jpg

Revision as of 19:59, 22 September 2008

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Preparation of pMPMT5-AID+AIDT7 construct
1. PCR - translation fusion: AID + T7 RNA-polimerase
Primers: AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
Annealing temperature: 55 °C
Annealing time: 4 minutes
two reapeats: 20 and 25 cycles
2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br

PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

Tranlacyjna_pcr_29_05_2008.jpg



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