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Team:Warsaw/Calendar-Main/30 June 2008
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<p>'''Preparation of OmpA protein fusions'''<br> | <p>'''Preparation of OmpA protein fusions'''<br> | ||
| - | 1. PCR on pBD (jaki numerek?) plasmid with ompaL_N and ompaP_link primers <br> | + | 1. PCR on pBD (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (15 cycles, annealing length 45s, annealing temperature 63 degrees)<br> |
| - | 2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers <br> | + | 2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (20 cycles, annealing length 45s, annealing temperature 63 degrees)<br> |
| - | 3. PCR on pUC19 plasmid with </p> | + | 3. PCR on pUC19 plasmid with omegaL_link and omegaP_EPB primers (20 cycles, annealing length 30s, annealing temperature 58 degrees)<br> |
| + | As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega. <br> | ||
| + | 4. Gel electrphoresis of PCR products and gel-out of proper bands</p> | ||
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Preparation of OmpA protein fusions
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