Team:Warsaw/Calendar-Main/30 June 2008

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<p>'''Optimisation of primers from 19.06'''<br></p>
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<p>'''Optimisation of primers from 19.06'''<br>
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1. PCR on pBD (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (annealing length 45s)<br>
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2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (annealing length 45s)<br>
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3. PCR on pUC19 plasmid with omegaL_link and omegaP_EPB primers (annealing length 30s)<br>
 +
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p>

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Optimisation of primers from 19.06
1. PCR on pBD (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (annealing length 45s)
2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (annealing length 45s)
3. PCR on pUC19 plasmid with omegaL_link and omegaP_EPB primers (annealing length 30s)
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees