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Team:Warsaw/Calendar-Main/2 July 2008
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MKrzyszton (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Preparation of constructs with OmpA protein fusions'''<br> </p> <!-- do not remove this! --> {{WarNotebookEnd}}) |
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<p>'''Preparation of constructs with OmpA protein fusions'''<br> | <p>'''Preparation of constructs with OmpA protein fusions'''<br> | ||
| + | 1. PCR on OmpA (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (15 cycles, annealing length 45s, annealing temperature 63 degrees) | ||
| + | 2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (20 cycles, annealing length 45s, annealing temperature 63 degrees) | ||
</p> | </p> | ||
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Preparation of constructs with OmpA protein fusions
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