Team:Warsaw/Calendar-Main/2 July 2008

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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
<p>'''Preparation of constructs with OmpA protein fusions'''<br>
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1. PCR on OmpA (jaki numerek?) plasmid with ompaL_N and ompaP_link primers (15 cycles, annealing length 45s, annealing temperature 63 degrees)
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1. PCR on OmpA_linker and linker_alpha  with ompaL_N and alphaP_XB primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)
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2. PCR on pUC19 plasmid with alphaL_link and alphaP_XB primers (20 cycles, annealing length 45s, annealing temperature 63 degrees)
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2. PCR on OmpA_linker and linker_omega  with ompaL_N and alphaP_XB primers (20 cycles, annealing length 45s, annealing temperature 63 degrees)
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Revision as of 18:59, 23 September 2008

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Preparation of constructs with OmpA protein fusions
1. PCR on OmpA_linker and linker_alpha with ompaL_N and alphaP_XB primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees) 2. PCR on OmpA_linker and linker_omega with ompaL_N and alphaP_XB primers (20 cycles, annealing length 45s, annealing temperature 63 degrees)



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