Team:Warsaw/Calendar-Main/26 June 2008

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(Difference between revisions)
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<p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br>
<p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br>
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1. Transformation of E. coli strain TOP10 with ligation from previous day <br>
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1. Transformation of E. coli TOP10 strain with ligation from previous day <br>
2. Transformants plating on LB + tetracycline</p>
2. Transformants plating on LB + tetracycline</p>

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Preparation of pMPMT5-AID+AIDT7 construct
1. Transformation of E. coli TOP10 strain with ligation from previous day
2. Transformants plating on LB + tetracycline