Team:Warsaw/Calendar-Main/3 July 2008

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(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Preparation of constructs with OmpA protein fusions'''<br> 1. Clean-up of overnight digestation <br> 2. Ligation of OmpA_alfa and pE...)
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3. Ligation of OmpA_omega and pET15b<br>
3. Ligation of OmpA_omega and pET15b<br>
4. Transformation of E. coli TOP10 strain with these ligations <br></p>
4. Transformation of E. coli TOP10 strain with these ligations <br></p>
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5. Transformants plating on LB + ampicillin
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Revision as of 19:23, 23 September 2008

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Preparation of constructs with OmpA protein fusions
1. Clean-up of overnight digestation
2. Ligation of OmpA_alfa and pET15b
3. Ligation of OmpA_omega and pET15b
4. Transformation of E. coli TOP10 strain with these ligations

5. Transformants plating on LB + ampicillin </p>