Team:Warsaw/Calendar-Main/3 July 2008

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2. Ligation of OmpA_alfa and pET15b <br>
2. Ligation of OmpA_alfa and pET15b <br>
3. Ligation of OmpA_omega and pET15b<br>
3. Ligation of OmpA_omega and pET15b<br>
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4. Transformation of E. coli TOP10 strain with these ligations <br></p>
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4. Transformation of E. coli TOP10 strain with these ligations <br>
5. Transformants plating on LB + ampicillin
5. Transformants plating on LB + ampicillin
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Preparation of constructs with OmpA protein fusions
1. Clean-up of overnight digestation
2. Ligation of OmpA_alfa and pET15b
3. Ligation of OmpA_omega and pET15b
4. Transformation of E. coli TOP10 strain with these ligations
5. Transformants plating on LB + ampicillin