Team:Warsaw/Calendar-Main/7 July 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Preparation of constructs with OmpA protein fusions''' <br> 1. Digestation of confirmed clones of </p> <!-- do not remove this...)
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<p>'''Preparation of constructs with OmpA protein fusions''' <br>
<p>'''Preparation of constructs with OmpA protein fusions''' <br>
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1. Digestation of confirmed clones of  
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1. Digestation of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI<br>
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2. Digestation of pACYC177 plasmid with NdeI and BamHI<br>
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3. Gel electrophoresis and digested plasmids and gel-out of proper bands
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</p>

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Preparation of constructs with OmpA protein fusions
1. Digestation of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI
2. Digestation of pACYC177 plasmid with NdeI and BamHI
3. Gel electrophoresis and digested plasmids and gel-out of proper bands