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Team:Warsaw/Calendar-Main/7 July 2008
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<p>'''Preparation of constructs with OmpA protein fusions''' <br> | <p>'''Preparation of constructs with OmpA protein fusions''' <br> | ||
1. Digestation of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI<br> | 1. Digestation of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI<br> | ||
| - | 2. Digestation of pACYC177 plasmid with NdeI and BamHI<br> | + | 2. Digestation of pACYC177 plasmid with NdeI and BamHI, dephosphorylation with CIAP<br> |
| - | 3. Gel electrophoresis and digested plasmids and gel-out of proper bands | + | 3. Gel electrophoresis and digested plasmids and gel-out of proper bands <br> |
| + | 4. Overnight ligation of pACYC177 and pET15b+OmpA_alpha<br> | ||
| + | 5. Overnight ligation of pACYC177 and pET15b+OmpA_omega | ||
</p> | </p> | ||
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Preparation of constructs with OmpA protein fusions
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