Team:NTU-Singapore/Notebook/25 September 2008
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(New page: =Thursday 25 September= *Minipreps for LsrA-YFP, followed by digestional gel check. *Make LuxS mutants more competent. *Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take ...)
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(New page: =Thursday 25 September= *Minipreps for LsrA-YFP, followed by digestional gel check. *Make LuxS mutants more competent. *Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take ...)
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Revision as of 03:35, 25 September 2008
Thursday 25 September
- Minipreps for LsrA-YFP, followed by digestional gel check.
- Make LuxS mutants more competent.
- Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take the supernatant (which contains AI-2), store at -20 degrees C.