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Team:Warsaw/Calendar-Main/7 July 2008
From 2008.igem.org
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2. Digestation of pACYC177 plasmid with NdeI and BamHI, dephosphorylation with CIAP<br> | 2. Digestation of pACYC177 plasmid with NdeI and BamHI, dephosphorylation with CIAP<br> | ||
3. Gel electrophoresis and digested plasmids and gel-out of proper bands <br> | 3. Gel electrophoresis and digested plasmids and gel-out of proper bands <br> | ||
| - | 4. Overnight ligation of pACYC177 and | + | 4. Overnight ligation of pACYC177 and OmpA_alpha<br> |
| - | 5. Overnight ligation of pACYC177 and | + | 5. Overnight ligation of pACYC177 and OmpA_omega |
</p> | </p> | ||
Revision as of 12:19, 26 September 2008
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Preparation of constructs with OmpA protein fusions
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