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Team:Warsaw/Calendar-Main/2 June 2008
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E.szczesna (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p>1. Transformation of E.coli TOP10 with pZC320<br> 2. LB + ampicillin(30μg/mL) + X-gal + IPTG<br> 3. PCR for AID transcription fusion...) |
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| - | <p> | + | <p>Piotr: Transformation of E.coli TOP10 with pZC320.</p> |
| - | + | <p>Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG</p> | |
| - | + | <h4>PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase</h4><p> | |
Primers: AIDlNrH and T7pXbSal<br> | Primers: AIDlNrH and T7pXbSal<br> | ||
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Time: 4 minutes<br> | Time: 4 minutes<br> | ||
| - | 20 cycles | + | 20 cycles |
Revision as of 13:16, 26 September 2008
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Piotr: Transformation of E.coli TOP10 with pZC320. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Primers: AIDlNrH and T7pXbSal Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5) Annealing temperature: 55°C Time: 4 minutes 20 cycles
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