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Team:Warsaw/Calendar-Main/2 June 2008
From 2008.igem.org
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Piotr: Transformation of E.coli TOP10 with pZC320. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Primers: AIDlNrH and T7pXbSal Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5) Annealing temperature: 55°C Time: 4 minutes Number of cycles: 20
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