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Team:Warsaw/Calendar-Main/3 June 2008
From 2008.igem.org
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| - | <p> | + | <p>DNA (PCR products) gel electrophoresis and isolation of DNA from proper bands<br> |
| - | + | Digestion of DNA with HindIII and SalI<br> | |
| - | + | Digestion of pMPM-T5 + AID with HindIII and SalI<br> | |
| - | + | Clean-up of products of p.2 and p.3 reactions<br> | |
| - | + | Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion)<br> | |
| - | + | Transformation of E.coli TOP10 with ligation product<br> | |
| - | + | Plating transformants on LB+tetracycline<br> | |
| - | + | Digestion of pMPM-T5 + transcription fusion with EcoRHI and HindIII<br> | |
| - | + | Use of T4 polymerase for blunting<br> | |
| - | + | DNA gel electrophoresis<br> | |
| - | + | Isolation of DNA from proper band<br> | |
| - | + | Ligation of product p.11 (pMPM-T5 + T7 RNA-polymerase)<br> | |
| - | + | Transformation of E.coli TOP10 with ligation product<br> | |
| - | + | Plating transformants on LB + tetracycline</p> | |
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Revision as of 13:29, 26 September 2008
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DNA (PCR products) gel electrophoresis and isolation of DNA from proper bands
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