Team:Warsaw/Calendar-Main/10 July 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Preparation of constructs with OmpA protein fusions'''<br> 1. Isolation of plasmids from cultures inocluated on previous day <br> 2....)
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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
<p>'''Preparation of constructs with OmpA protein fusions'''<br>
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1. Isolation of plasmids from cultures inocluated on previous day <br>
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1. Isolation of plasmids from cultures inocluated on previous day. <br>
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2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega)
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2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating. <br>
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3.
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Preparation of constructs with OmpA protein fusions
1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating.
3.