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Team:Warsaw/Calendar-Main/10 July 2008
From 2008.igem.org
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1. Isolation of plasmids from cultures inocluated on previous day. <br> | 1. Isolation of plasmids from cultures inocluated on previous day. <br> | ||
2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating. <br> | 2. Control digestation of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega). We didn't obtain pCACYC177 + OmpA_alpha probably because mistake in plating. <br> | ||
| - | 3. | + | 3. Ligation of pACYC177 and OmpA_alpha (1 hr)<br> |
</p> | </p> | ||
Revision as of 13:38, 26 September 2008
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Preparation of constructs with OmpA protein fusions
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