Team:Warsaw/Calendar-Main/10 June 2008

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
-
<p>Plasmid isolation from colonies.<br>
+
<p>Plasmid isolation from colonies.<br>
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>

Revision as of 13:50, 26 September 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



Plasmid isolation from colonies.
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
Control digestion of pMPM-T5 + T7 RNA-polymerase with EcoRI.
Control digestion of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
DNA gel electrophoresis of digestion products.
Picking out proper colonies of transformants.