Team:Warsaw/Calendar-Main/10 June 2008

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<p>Plasmid isolation from colonies.<br>
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<p>Plasmid isolation from night culture of our transformants.<br>
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>

Revision as of 13:53, 26 September 2008

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Plasmid isolation from night culture of our transformants.
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
Control digestion of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
Control digestion of pMPM-T5 + T7 RNA-polymerase with EcoRI.
Control digestion of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
DNA gel electrophoresis of digestion products.
Picking out proper colonies of transformants.

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