Team:Warsaw/Calendar-Main/16 June 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>Colony PCR<br> Template: DNA from white colonies<br> primers: PZCseqL and PZCseqR<br> electrophoresis of PCR products<br> gelout of...)
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<p>Colony PCR<br>
<p>Colony PCR<br>
-
Template: DNA from white colonies<br>
+
Template: DNA isolated from white colonies<br>
-
primers: PZCseqL and PZCseqR<br>
+
Primers: PZCseqL and PZCseqR<br>
-
electrophoresis of PCR products<br>
+
Annealing time:
-
gelout of proper products (~1200 bp)<br>
+
Number of cycles:
-
sequencing of gel-outed fragments using primer PZCseqL </p>
+
<p>DNA gel electrophoresis of PCR products.<br>
 +
 
 +
<p>Gel-out of proper products (~1200 bp).<br>
 +
 
 +
<p>Sequencing of proper fragments using primer PZCseqL.</p>

Revision as of 14:04, 26 September 2008

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Colony PCR
Template: DNA isolated from white colonies
Primers: PZCseqL and PZCseqR
Annealing time: Number of cycles: <p>DNA gel electrophoresis of PCR products.
<p>Gel-out of proper products (~1200 bp).
<p>Sequencing of proper fragments using primer PZCseqL.