Team:Warsaw/Calendar-Main/19 June 2008

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<p>Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:</p>
<p>Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:</p>
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<p>alphaL+link14+homo (alphaL_link)<br> CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG<br>
 
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alphaP+STOP+XbaI+BamHI (alphaP_XB)<br> GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG<br>
 
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omegaL+link14+homo (omegaL_link)<br> CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG<br>
 
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omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)<br> AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG<br>
 
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OmpAL+NdeI (ompaL_N)<br> CATATGAAAGCTACTAAACTGGTACTGGG<br>
 
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OmpAP+link8+SacI+NotI+homo (ompaP_link)<br> GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG</p>
 
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<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#alphaL+link14+homo (alphaL_link)">alphaL+link14+homo (alphaL_link)</a>
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<br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#alphaP+STOP+XbaI+BamHI (alphaP_XB)">alphaP+STOP+XbaI+BamHI (alphaP_XB)</a><br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#omegaL+link14+homo (omegaL_link)">omegaL+link14+homo (omegaL_link)</a><br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)">omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)</a><br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpAL+NdeI (ompaL_N)">OmpAL+NdeI (ompaL_N)</a> <br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpAP+link8+SacI+NotI+homo (ompaP_link)">OmpAP+link8+SacI+NotI+homo (ompaP_link)</p><br>
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</html>

Revision as of 14:14, 26 September 2008

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Primers for fusion of protein A and ompA and alpha or omega part of beta-lactamase were designed:

alphaL+link14+homo (alphaL_link)
alphaP+STOP+XbaI+BamHI (alphaP_XB)
omegaL+link14+homo (omegaL_link)
omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)
OmpAL+NdeI (ompaL_N)
OmpAP+link8+SacI+NotI+homo (ompaP_link)