Team:Warsaw/Calendar-Main/24 June 2008

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1. PCR - translation fusion: AID + T7 RNA-polimerase <br>
1. PCR - translation fusion: AID + T7 RNA-polimerase <br>
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Primers: AIDlNrH and T7pXbSal<br>
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Primers:  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br>
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Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion<br>
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion<br>

Revision as of 14:15, 26 September 2008

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Preparation of pMPMT5-AID+AIDT7 construct
1. PCR - translation fusion: AID + T7 RNA-polimerase
Primers: AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
Annealing temperature: 55 °C
Annealing time: 4 minutes
two reapeats: 20 and 25 cycles
2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br

PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

Tranlacyjna_pcr_29_05_2008.jpg



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