Wiki/Team:Warsaw/primers

From 2008.igem.org

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<h4>Fusion of protein A and ompA and alpha or omega part of beta-lactamase</h4>
<h4>Fusion of protein A and ompA and alpha or omega part of beta-lactamase</h4>
<pre>
<pre>
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<a name="alphaL+link14+homo (alphaL_link)">alphaL+link14+homo (alphaL_link)</a>          5' CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG 3'
+
<a name="AlphaL_link">alphaL+link14+homo (alphaL_link)</a>          5' CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG 3'
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<a name="alphaP+STOP+XbaI+BamHI (alphaP_XB)">alphaP+STOP+XbaI+BamHI (alphaP_XB)</a>        5' GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG 3'
+
<a name="AlphaP_XB">alphaP+STOP+XbaI+BamHI (alphaP_XB)</a>        5' GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG 3'
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<a name="omegaL+link14+homo (omegaL_link)">omegaL+link14+homo (omegaL_link)</a>          5' CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG 3'
+
<a name="OmegaL_link">omegaL+link14+homo (omegaL_link)</a>          5' CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG 3'
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<a name="omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)">omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)</a> 5' AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG 3'
+
<a name="OmegaP_EPB">omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)</a> 5' AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG 3'
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<a name="OmpAL+NdeI (ompaL_N)">OmpAL+NdeI (ompaL_N)</a>                      5' CATATGAAAGCTACTAAACTGGTACTGGG 3'
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<a name="OmpaL_N">OmpAL+NdeI (ompaL_N)</a>                      5' CATATGAAAGCTACTAAACTGGTACTGGG 3'
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<a name="OmpAP+link8+SacI+NotI+homo (ompaP_link)">OmpAP+link8+SacI+NotI+homo (ompaP_link)</a>  5' GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG 3'
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<a name="OmpaP_link">OmpAP+link8+SacI+NotI+homo (ompaP_link)</a>  5' GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG 3'
</pre>
</pre>
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Revision as of 14:18, 26 September 2008

Gallery Bricks Notebook Team Project Home

 

Primers

One-armed bandit preparation

AID and T7 cloning

T7lLinkB   5' CGGATCCGGCGGTGGCGGTAGTGGAGGAGGTGGGTCTGGAGGCGGAGGGAACACGATTAACATCGCTAAGAAC  3'
T7lRBSHi   5' CACAAGCTTAGGAGGTGTACTAGATGAACACGATTAACATCGCTAAG  3'
T7pXbSal   5' TCTGTCGACTCTAGATTACGCGAACGCGAAGTCC  3'
AIDlNrH    5' CGTAAGCTTAGGAGGTGTAGACCATGGACAGCCTCTTGATG  3'
AIDpLinB   5' CCACTACCGCCACCGCCGGATCCGCCACCTCCGCTGCCTCCACCACCAAGTCCCAAAGTACGAAATGC  3'

Sequencing of pMPM-T5

pMPMT5seqF  5' CTACTGTTTCTCCATACCCG  3'
pMPMT5seqR  5' GAAAATCTTCTCTCATCCGC 3'
AIDseqP     5' GCTTCGGCGCATCCTTTTG 3'

Sequencing of β-galactosidase from pZC320

pZCseqL     5' CTTGACCCGCAGTTGCAAAC 3'
pZCseqP     5' CCCAACTGATCTTCAGCATC 3'

Hunter and prey

Fusion of protein A and ompA and alpha or omega part of beta-lactamase

alphaL+link14+homo (alphaL_link)          5' CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CACCCAGAAACGCTGGTGAAAG 3'
alphaP+STOP+XbaI+BamHI (alphaP_XB)        5' GG GGATCC GG TCTAGA TTA TTCGCCAGTTAATAGTTTGCG 3'
omegaL+link14+homo (omegaL_link)          5' CAGTGGTGGAAGCGGTGGC GGAGGATCTGGAGGAGGTGGTTCTGGAGGTGGAGGT CTACTTACTCTAGCTTCCCGG 3'
omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB) 5' AAGGATCCAACTGCAGAAGAATTC TTACCAATGCTTAATCAGTG 3'
OmpAL+NdeI (ompaL_N)                      5' CATATGAAAGCTACTAAACTGGTACTGGG 3'
OmpAP+link8+SacI+NotI+homo (ompaP_link)   5' GCCACCGCTTCCACCACT GCGGCCGC CA GAGCTC CCTCCTCCAGAACCTCC GCCGTTGTCCGGACGAGTGCCGATG 3'




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