Team:Warsaw/Calendar-Main/24 June 2008

From 2008.igem.org

(Difference between revisions)
Line 7: Line 7:
'''Preparation of pMPMT5-AID+AIDT7 construct'''<br>
'''Preparation of pMPMT5-AID+AIDT7 construct'''<br>
-
1. PCR - translation fusion: AID + T7 RNA-polimerase <br>
+
PCR - translation fusion: AID + T7 RNA-polimerase <br>
Primers:  
Primers:  
Line 21: Line 21:
two reapeats: 20 and 25 cycles<br>
two reapeats: 20 and 25 cycles<br>
-
2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br
+
Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br
<h3>PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>
<h3>PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.<br> Lane 1 - 25 cycles, lane 2 - 20 cycles.</h3>

Revision as of 14:19, 26 September 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of pMPMT5-AID+AIDT7 construct
PCR - translation fusion: AID + T7 RNA-polimerase
Primers: AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
Annealing temperature: 55 °C
Annealing time: 4 minutes
two reapeats: 20 and 25 cycles
Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles) ,br

PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct.
Lane 1 - 25 cycles, lane 2 - 20 cycles.

Tranlacyjna_pcr_29_05_2008.jpg



Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/24_June_2008"