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Team:Warsaw/Calendar-Main/30 June 2008
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<p>'''Optimisation of primers from 19.06'''<br> | <p>'''Optimisation of primers from 19.06'''<br> | ||
| - | 1. PCR on pBD (jaki numerek?) plasmid with | + | 1. PCR on pBD (jaki numerek?) plasmid with |
| - | 2. PCR on pUC19 plasmid with | + | <html> |
| - | 3. PCR on pUC19 plasmid with | + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> |
| + | </html> | ||
| + | primers (annealing length 45s)<br> | ||
| + | 2. PCR on pUC19 plasmid with | ||
| + | <html> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a> | ||
| + | </html> | ||
| + | primers (annealing length 45s)<br> | ||
| + | 3. PCR on pUC19 plasmid with | ||
| + | <html> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> | ||
| + | </html> | ||
| + | primers (annealing length 30s)<br> | ||
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p> | Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees</p> | ||
Revision as of 14:25, 26 September 2008
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Optimisation of primers from 19.06
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