Team:Warsaw/Calendar-Main/11 July 2008

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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
<p>'''Preparation of constructs with OmpA protein fusions'''<br>
-
1. Colony PCR on colonies from plates with transformations OmpA_alpha <br>
+
1. Colony PCR on colonies from plates with transformations OmpA_alpha. <br>
-
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin</p>
+
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.<br>
 +
'''Cloning of protein Z DNA to OmpA constructs''' <br>
 +
1. 2 colonies was inoculated to liquid LB broth with kanamycin
 +
</p>

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Preparation of constructs with OmpA protein fusions
1. Colony PCR on colonies from plates with transformations OmpA_alpha.
2. Confirmed transformant colonies inoculated to liquid LB with kanamycin.
Cloning of protein Z DNA to OmpA constructs
1. 2 colonies was inoculated to liquid LB broth with kanamycin