Team:Warsaw/Calendar-Main/12 July 2008

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<p>'''Preparation of constructs with OmpA protein fusions'''and '''Cloning of protein Z DNA to OmpA constructs''' <br>
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<p>'''Preparation of constructs with OmpA protein fusions''' and '''Cloning of protein Z DNA to OmpA constructs''' <br>
1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha and pACYC177+OmpA_Z_omega). <br>
1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha and pACYC177+OmpA_Z_omega). <br>

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Preparation of constructs with OmpA protein fusions and Cloning of protein Z DNA to OmpA constructs
1. Isolation of plasmids from cultures inocluated on previous day (pCACYC177 + OmpA_alpha and pACYC177+OmpA_Z_omega).
2. Control digestation of isolated plasmids with BamHI and SacI (we found good clones for both ligations).

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