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Team:Warsaw/Calendar-Main/14 July 2008
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1. Digestation and dephosphorylatio (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. <br> </p> | 1. Digestation and dephosphorylatio (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. <br> </p> | ||
2. Gel eloctrophoresis and gel-out (4300 bp band). <br> | 2. Gel eloctrophoresis and gel-out (4300 bp band). <br> | ||
| - | 3. Ligation of isolated DNA fragment and Z DNA fragment isolated on | + | 3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. <br> |
| + | 4. | ||
Revision as of 15:19, 26 September 2008
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Cloning of protein Z DNA to OmpA constructs 2. Gel eloctrophoresis and gel-out (4300 bp band).
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