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Team:Warsaw/Calendar-Main/14 July 2008
From 2008.igem.org
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2. Gel eloctrophoresis and gel-out (4300 bp band). <br> | 2. Gel eloctrophoresis and gel-out (4300 bp band). <br> | ||
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. <br> | 3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. <br> | ||
| - | 4. | + | 4. Transformation of E. coli TOP10 strain with ligation. <br> |
| + | 5. Transformants plating on LB + kanamycin. | ||
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Cloning of protein Z DNA to OmpA constructs 2. Gel eloctrophoresis and gel-out (4300 bp band).
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