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Team:Warsaw/Calendar-Main/17 July 2008
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<p>'''Cloning of protein A DNA to OmpA constructs'''<br> | <p>'''Cloning of protein A DNA to OmpA constructs'''<br> | ||
| - | 1. Digestion of pKS-A plasmid with NotI and SacI <br> | + | 1. Digestion of pKS-A plasmid, pACYC177+OmpA_omega and pACYC177+OmpA_alpha with NotI and SacI (pACYC177 were also dephosporylated with CIAP)<br> |
| + | 2. Gel electrophoresis and gel-out of proper bands (420 bp - pKS-A lane, 4050 bp - pACYC177+OmpA_omega lane and 4300 bp pACYC177+OmpA_alpha lane). <br> | ||
| + | 3. Ligation of A DNA fragment with both pACYC177 vectors. <br> | ||
</p> | </p> | ||
Revision as of 16:31, 26 September 2008
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Cloning of protein A DNA to OmpA constructs
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