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Team:Warsaw/Calendar-Main/27 May 2008
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| + | <p>Michał K.:</p> | ||
| + | <p>1. <a href:"https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcription fusion</p> | ||
| - | + | <p>2. <a href:"https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion</p> | |
| - | + | <p>3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI</p> | |
| - | + | <p>4. Control digest of pMPM-T5 with translation fusion with BamHI</p> | |
| - | + | <p>5. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI</p> | |
| - | + | <p>6. Choice of proper clones and preparation for sequencing</p> | |
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| - | 6. Choice of proper clones and preparation for sequencing | + | |
<h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | <h3>Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | ||
Digestion was conducted with BamHI</h3> | Digestion was conducted with BamHI</h3> | ||
| + | </html> | ||
<center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center> | <center>https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg</center> | ||
Revision as of 17:58, 26 September 2008
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Michał K.: 1. Isolation of hypothetical pMPM-T5 with transcription fusion 2. Isolation of hypothetical pMPM-T5 with translation fusion 3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI 4. Control digest of pMPM-T5 with translation fusion with BamHI 5. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI 6. Choice of proper clones and preparation for sequencing Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
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